Agrobacterium rhizogenes-mediated transformation of Superroot-derived Lotus corniculatus plants: a valuable tool for functional genomics
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Agrobacterium rhizogenes-Mediated Transformation of the Parasitic Plant Phtheirospermum japonicum
BACKGROUND Plants within the Orobanchaceae are an agriculturally important group of parasites that attack economically important crops to obtain water and nutrients from their hosts. Despite their agricultural importance, molecular mechanisms of the parasitism are poorly understood. METHODOLOGY/PRINCIPAL FINDINGS We developed transient and stable transformation systems for Phtheirospermum jap...
متن کاملAgrobacterium rhizogenes - Mediated Transformation of Medicinal Plants from the Family Rhamnaceae
A number of plants can be successfully transformed by Agrobacterium rhizogenes through the transfer of T-DNA from agrobacteria to the plant genome. Transgenic tissue – hairy roots – is produced as a result of the transformation process. This organized, genetically stable, hormone-independent transformed tissue is capable of accomplishing complex metabolic pathways, including biosynthesis and ac...
متن کاملAgrobacterium Rhizogenes-mediated Transformation of Peganum multisectum (Maxim) Bobrov and Harmine Production in Hairy Roots
Using Agrobacterium rhizogenes due to create hairy roots is a useful method to product secondary metabolites in many medicinal plants. The transgenic hairy roots were induced from Peganum multisectum (Maxim) Bobrov a medicinally important species, by infecting leaf and stem explant with wild type Agrobacterium rhizogenes strain ATTCC 15834, which led to the induction of hairy roots from 19% of ...
متن کاملFactors Affecting Agrobacterium-mediated Transformation of Plants
Plant transformation technology has become a versatile platform for cultivar improvement as well as for studying gene function in plants. The development of an efficient method for genetic transformation is a prerequisite for the application of molecular biology to the improvement of a given crop species. Agrobacterium-mediated genetic transformation is the dominant technology used for the prod...
متن کاملThe Agrobacterium rhizogenes GALLS gene encodes two secreted proteins required for genetic transformation of plants.
Agrobacterium tumefaciens and Agrobacterium rhizogenes are related pathogens that cause crown gall and hairy root diseases, which result from integration and expression of bacterial genes in the plant genome. Single-stranded DNA (T strands) and virulence proteins are translocated into plant cells by a type IV secretion system. VirD2 nicks a specific DNA sequence, attaches to the 5' end, and pil...
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ژورنال
عنوان ژورنال: BMC Plant Biology
سال: 2009
ISSN: 1471-2229
DOI: 10.1186/1471-2229-9-78